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AmpiDots

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Exponential Amplification and Rapid Detection of miRNAs using DNA Quantum Dot Bi...

Exponential Amplification and Rapid Detection of miRNAs using DNA Quantum Dot Bioconjugates for Disease Diagnostics Accurate and timely diagnosis is critical in the treatment of all diseases. Researchers are constantly discovering new links between specific biomolecules and diseases, often uncovering ‘panels’ of markers with high sensitivity an... see more

31/12/2018

ETH Zürich

187K€

Project Budget: 187K€

Project leader

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH No se ha especificado una descripción o un objeto social para esta compañía.

PARTICIPATION DEPralty Sin fecha límite de participación.

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Financing granted El organismo H2020 notifico la concesión del proyecto The day 2018-12-31

MSCA-IF-2015-EF: Marie Skłodowska-Curie Individual F... Scope:Proposals for European Fellowships involve a single host organisation (future beneficiary) est...

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Este proyecto no cuenta con búsquedas de partenariado abiertas en este momento.

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2 Participantes

ETH Zürich

187.42K€ | Leader

ARGAL ALIMENTACION

229.54K€ | participant

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Project duration: 33 months Date Start: 2016-03-21
End date: 2018-12-31

Project leader

EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH No se ha especificado una descripción o un objeto social para esta compañía.

Project Budget 187K€

participation deadline Sin fecha límite de participación.

Project description Accurate and timely diagnosis is critical in the treatment of all diseases. Researchers are constantly discovering new links between specific biomolecules and diseases, often uncovering ‘panels’ of markers with high sensitivity and specificity for disease diagnostics. However, our current diagnostic tools are unable to take advantage of these panels as they lack sensitivity, or are too costly and slow for practical application. Accordingly, new technologies that can help transform these fundamental discoveries into revolutionary clinical tools are in high demand. These must be rapid, efficient, robust and cost-effective. To this end, I will develop a new rapid diagnostic assay that combines the unique functional properties of fluorescent nanomaterials and enzymes. The core technology is an advanced nanoparticle-biomolecule construct consisting of highly fluorescent ‘quantum dot’ inorganic nanoparticles coated with DNA. These nanobioconjugates (termed ‘AmpiDots’) act simultaneously as both templates for isothermal amplification of target miRNAs and as highly sensitive fluorescent sensors. The result will be a rapid, highly sensitive, versatile and efficient assay that can detect multiple targets simultaneously and give a robust signal for assay readout. A critical aspect of the work will be the use of advanced microfluidic-based analytical systems to optimise the assay and to study the unique phenomena that arise when enzymes interact with nanoparticles.

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