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LOW-ENERGY ELECTRONS

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Cerrado

Experimental investigation of physico chemical effects of low energy electron in...

Experimental investigation of physico chemical effects of low energy electron interaction with isolated clustered and self assembled biomolecules The proposed study will focus on effects of low-energy electron interaction with isolated amino acids and short peptides deposited on lipid bilayers mimicking the cellular membrane, as well as on oligonucleotides and on DNA. Owing... ver más

29/02/2012

100K€

Presupuesto del proyecto: 100K€

Líder del proyecto

THE OPEN UNIVERSITY No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo FP7 notifico la concesión del proyecto el día 2012-02-29 No tenemos la información de la convocatoria

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1 Participantes

0.00€ | Lider

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Líder del proyecto

THE OPEN UNIVERSITY No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 100K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto The proposed study will focus on effects of low-energy electron interaction with isolated amino acids and short peptides deposited on lipid bilayers mimicking the cellular membrane, as well as on oligonucleotides and on DNA. Owing to the simple structure of phospholipids, they present a geometrically and physico-chemically well-defined biomolecular array and potentially an ideal matrix for homogenous distribution of amino-acids, nucleotides, DNA, and RNA chains, with or without presence of water. By finding the probability for the temporary electron attachment to amino acids, oligonucleotides and DNA molecules and observing and quantifying the effects of vibrational and/or electronic excitation when molecules are combined into more complex structure, the proposed research will be advancing the knowledge in Physics, Chemistry and Biology and will be relevant for nanotechnology in their quest for new materials and creation of electronic molecular devices. The analysis of damage products (radicals) from the interaction of electrons and ion radicals with short oligonucleotides and DNA deposited on lipid films in the presence of specific amino acids will be performed aiming to possibly tracking the path of proton transfer and oxygen radical in the substrate after irradiation by electrons. An important aspect of the proposed research is investigation of the role of water in the processes of resonance formation and fragmentation of lipids and other biomolecules deposited on a lipid substrate. Measurements will be performed using several experimental techniques: Electron Energy Loss spectroscopy (Time-of-Flight measurements of the spatial and temporal distribution of electrons reflected from the sample and transmitted current measurements), X-ray Photoelectron Spectroscopy (using micro-beams; synchrotron installation), High Performance Liquid Chromatography, and gel electrophoreses. Atomic Force Microscopy and Scanning Tunneling Microscopy images will be used in addition.

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