Liver Bioengineering
Financiado
Ex vivo Re vascularization in Porcine Liver Bioengineering A Critical First St...
Ex vivo Re vascularization in Porcine Liver Bioengineering A Critical First Step Towards Effective Transplantation of Bioengineered Livers
Liver transplantation is currently the only proven therapy to extend life of patients with terminal liver disease. Organ bioengineering and regenerative medicine are promising new technologies that can help reduce the burden of li...
Liver transplantation is currently the only proven therapy to extend life of patients with terminal liver disease. Organ bioengineering and regenerative medicine are promising new technologies that can help reduce the burden of liver shortage by increasing the number of organs available for transplantation. However, current bioengineered livers lack a functional vascular network that can readily allow their transplantation into a living host. Previous studies by the candidate have shown successful recellularization of acellular liver scaffolds using endothelial cells and hepatic cells in a perfusion bioreactor, creating a vascularized human liver organoid. However, its vascular network was unable to maintain vascular patency under constant blood flow for long periods of time. Hence, better understanding of how experimental cell seeding conditions of porcine liver scaffolds influence their re-vascularization efficiency is critical to achieve sustainable vascular patency after transplantation. In order to accomplish this, the impact of fluid flow pressure and the seeded cell number will be investigated in re-endothelialization efficiency of an acellular porcine liver scaffold. Furthermore, bioreactor pre-conditioning with fluid flow pressure ramping and sequential cycles of vascular growth and maturation will be used to induce re-vascularization, maturation, and enhanced function to potentially increase vascular patency. Finally, re-vascularized liver scaffolds will be transplanted into 5-10Kg pigs and short and long-term vascular patency will be investigated. Hence, the long-term objective of this project is to create a functional re-vascularized porcine liver scaffold, a critical first step towards the effective transplantation of bioengineered livers.
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Duración del proyecto: 27 meses
Fecha Inicio: 2015-04-07
Fecha Fin: 2017-07-31
Fecha Fin: 2017-07-31
Línea de financiación: concedida
El organismo H2020 notifico la concesión del proyecto el día 2017-07-31
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
MSCA-IF-2014-EF:
Marie Skłodowska-Curie Individual Fellowships (IF-EF)
Cerrada
hace 10 años
Presupuesto
El presupuesto total del proyecto asciende a
158K€
Líder del proyecto
Instituto Aragonés de Ciencias de la Salud
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
390K
Perfil tecnológico
TRL
4-5
390K