Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

VISCREEN

Financiado

Engineering viral vectors for high-throughput CRISPR genetic screens in plants

Tools based on CRISPR-Cas systems hold great potential both for fundamental plant genetics and precision crop breeding. Unlike traditional mutagenic approaches, CRISPR-Cas systems allow for the simultaneous delivery of thousands o... ver más

31/03/2025

KU Leuven

176K€

Presupuesto del proyecto: 176K€

Líder del proyecto

KATHOLIEKE UNIVERSITEIT LEUVEN No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Ver 2 Participantes

Financiación concedida El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-03-09

HORIZON-MSCA-2022-PF-01-01: MSCA Postdoctoral Fellowships 2022 ExpectedOutcome:

I+D

Cerrada hace 2 años

0% 100% 100%

2 Participantes

KU Leuven

154.42K€ | Lider

VLAAMS INSTITUUT BIOTECHNOL...

21.50K€ | Participante

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Proyectos interesantes

PID2020-114691RB-I00 BIOTECNOLOGIA DE VIRUS DE PLANTAS: VECTORES VIRALES Y ESTRAT... 272K€ Cerrado

RYC-2008-02529 Mecanismos de silenciamiento de RNA y de su supresión por su... 192K€ Cerrado

BES-2017-082729 APLICACIONES DEL SISTEMA CRISPR/CAS9 EN BIOTECNOLOGIA DE TRI... 93K€ Cerrado

OMEGA Developing technologies to engineer plant genomes at the meg... 1M€ Cerrado

BIO2017-92613-EXP ESTRATEGIAS DE SELECCION CONDICIONAL PARA IDENTIFICAR FENOTI... 52K€ Cerrado

PRE2020-095111 TRANSPOSONES, UNA FUENTE IMPORTANTE DE VARIABILIDAD GENETICA... 99K€ Cerrado

Duración del proyecto: 24 meses Fecha Inicio: 2023-03-09
Fecha Fin: 2025-03-31

Líder del proyecto

KATHOLIEKE UNIVERSITEIT LEUVEN No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 176K€

Descripción del proyecto Tools based on CRISPR-Cas systems hold great potential both for fundamental plant genetics and precision crop breeding. Unlike traditional mutagenic approaches, CRISPR-Cas systems allow for the simultaneous delivery of thousands of unique guide RNAs, enabling the generation of collections of higher-order mutants for rapid functional screens and the discovery of genetic interactions. However, CRISPR technology suffers from a ‘transformation bottleneck’; relatively few crop genotypes can be transformed and often only a handful of mutant lines can be produced. In virus-induced genome editing (VIGE), plant viruses are engineered to deliver gRNAs into cells. Such a capability is highly attractive for CRISPR knock-out (KO) screens as it could simplify the delivery and generate large populations of mutant lines in a broader range of crop genotypes. However, the application of VIGE for CRISPR KO screens is hampered by the lack of a proper viral-based multiplex gRNA delivery vector. My host lab has pioneered the development of CRISPR KO screens in plants, and in VISCREEN I will combine this knowledge with my expertise on VIGE to scale up CRISPR-Cas-mediated manipulation of plant genomes. The main objectives of my project are: (i) to develop Barley stripe mosaic virus (BSMV) for high-throughput CRISPR screens in wheat and maize; and (ii) to engineer and validate a set of viral vectors for VIGE in soybean. The viral vector-based, high-throughput editing systems developed in VISCREEN will expand CRISPR screens in a wider variety of crops and allow to test fundamental biological questions that are limited by functional redundancy. Overall, this innovative approach will make me a well-trained academic researcher with the capabilities to develop a professional career within the plant genome editing field.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores