VISCREEN
Financiado
Engineering viral vectors for high-throughput CRISPR genetic screens in plants
Tools based on CRISPR-Cas systems hold great potential both for fundamental plant genetics and precision crop breeding. Unlike traditional mutagenic approaches, CRISPR-Cas systems allow for the simultaneous delivery of thousands o...
Tools based on CRISPR-Cas systems hold great potential both for fundamental plant genetics and precision crop breeding. Unlike traditional mutagenic approaches, CRISPR-Cas systems allow for the simultaneous delivery of thousands of unique guide RNAs, enabling the generation of collections of higher-order mutants for rapid functional screens and the discovery of genetic interactions. However, CRISPR technology suffers from a ‘transformation bottleneck’; relatively few crop genotypes can be transformed and often only a handful of mutant lines can be produced. In virus-induced genome editing (VIGE), plant viruses are engineered to deliver gRNAs into cells. Such a capability is highly attractive for CRISPR knock-out (KO) screens as it could simplify the delivery and generate large populations of mutant lines in a broader range of crop genotypes. However, the application of VIGE for CRISPR KO screens is hampered by the lack of a proper viral-based multiplex gRNA delivery vector. My host lab has pioneered the development of CRISPR KO screens in plants, and in VISCREEN I will combine this knowledge with my expertise on VIGE to scale up CRISPR-Cas-mediated manipulation of plant genomes. The main objectives of my project are: (i) to develop Barley stripe mosaic virus (BSMV) for high-throughput CRISPR screens in wheat and maize; and (ii) to engineer and validate a set of viral vectors for VIGE in soybean. The viral vector-based, high-throughput editing systems developed in VISCREEN will expand CRISPR screens in a wider variety of crops and allow to test fundamental biological questions that are limited by functional redundancy. Overall, this innovative approach will make me a well-trained academic researcher with the capabilities to develop a professional career within the plant genome editing field.
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Duración del proyecto: 24 meses
Fecha Inicio: 2023-03-09
Fecha Fin: 2025-03-31
Fecha Fin: 2025-03-31
Línea de financiación: concedida
El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-03-09
Línea de financiación objetivo
El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2022-PF-01-01:
MSCA Postdoctoral Fellowships 2022
Cerrada
hace 2 años
Presupuesto
El presupuesto total del proyecto asciende a
176K€
Líder del proyecto
KATHOLIEKE UNIVERSITEIT LEUVEN
No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico
TRL
4-5
Perfil tecnológico
TRL
4-5