Innovating Works

StrainBooster

Financiado
Enforced ATP wasting as a general design principle to rationally engineer microb...
Enforced ATP wasting as a general design principle to rationally engineer microbial cell factories One global challenge of humanity in the 21st century is the shift from a petrochemical to a bio-based production of chemicals and fuels. An enabling technology towards this goal is metabolic engineering which uses computational an... One global challenge of humanity in the 21st century is the shift from a petrochemical to a bio-based production of chemicals and fuels. An enabling technology towards this goal is metabolic engineering which uses computational and experimental methods to construct microbial cell factories with desired properties. While it has been shown that genetically engineered microorganisms can, in principle, produce a broad range of chemicals, novel approaches to improve the performance of those strains are urgently needed to develop economically viable bioprocesses. To this end, we propose a new metabolic design principle to rationally engineer cell factories with high performance. Supported by a recent pilot study, we postulate that suitable genetic interventions combined with mechanisms that burn (waste) an extra amount of ATP (e.g., by artificial futile cycles) will increase product yield and productivity of many microbial production strains. Key objectives of StrainBooster are therefore: (1) to use computational techniques and metabolic models to identify gene knockout strategies whose coupling with ATP wasting mechanisms can boost the performance of microbial strains and to prove in silico that those strategies exist for many combinations of substrates, products, and host organisms; (2) to develop genetic modules that can robustly increase ATP dissipation in the cell; and (3) to experimentally demonstrate the power of the proposed strategy for selected production processes with Escherichia coli. To reach these ambitious goals, an interdisciplinary approach will be pursued combining theoretical and experimental studies and making use of innovative methods from systems and synthetic biology. If successful, StrainBooster will not only establish a new and ground-breaking strategy for metabolic engineering, it will also deliver novel computational tools and genetic parts facilitating direct application of the approach to design and optimize industrial fermentation processes. ver más
31/12/2022
MPG
2M€
Duración del proyecto: 70 meses Fecha Inicio: 2017-02-20
Fecha Fin: 2022-12-31

Línea de financiación: concedida

El organismo H2020 notifico la concesión del proyecto el día 2022-12-31
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
ERC-2016-COG: ERC Consolidator Grant
Cerrada hace 8 años
Presupuesto El presupuesto total del proyecto asciende a 2M€
Líder del proyecto
MAXPLANCKGESELLSCHAFT ZUR FORDERUNG DER WISSE... No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5