Developement of robust and quantitative biosensors based on near infrared two dy...
Developement of robust and quantitative biosensors based on near infrared two dyed silicate nanoparticles
The aim of this project is to develop quantitative biological sensors based on fluorescent spherical silicate nanoparticles (amorphous silica, zeolite beta) in controlled sizes in the range of 50 to 200 nanometers in diameter. The...
ver más
¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video
Proyectos interesantes
Photosense
Photoacoustic and fluorescence imaging platform for biosensi...
195K€
Cerrado
MAT2011-25870
NANOMATERIALES CON ALTA CAPACIDAD DE RECONOCIMIENTO MODULABL...
150K€
Cerrado
NanoPD
Single Molecule Nanoscale Sensors for Improved Therapies and...
2M€
Cerrado
FJC2020-044608-I
The use of near-infrared-emitting nanoparticles in biomedic...
53K€
Cerrado
MAT2012-38573-C02-02
UNIVERSAL DIAGNOSTIC PLATFORMS BASED ON OLIGONUCLEOTIDE CODI...
112K€
Cerrado
NanoPorous DNA-array
Ultra high density three dimentional DNA arrays for biosensi...
187K€
Cerrado
Fecha límite de participación
Sin fecha límite de participación.
Descripción del proyecto
The aim of this project is to develop quantitative biological sensors based on fluorescent spherical silicate nanoparticles (amorphous silica, zeolite beta) in controlled sizes in the range of 50 to 200 nanometers in diameter. The quantification of the analyte is based on a ratiometric detection of fluorescence from two dyes. The dyes are chosen such that the fluorescence of one dye is a function of an analyte (ion, biomolecule) concentration (sensing dye) whereas the fluorescence of the other dye is independent of variations in the medium (reference dye). Both dyes have the near-infrared light absorption property which lead to significant improvements for the detection in biological samples. The first step consists in confining a dye in the inorganic network yielding highly brilliant and photostable objects. Thus this fluorescent hybrid core can be used as the reference dye. Afterwards the ability of such material to undergo further chemical modification through the surface silanol functions and the use of coupling agents allows the immobilization of the sensing dye (commercially available or developed in the host structure). This is considered through various strategies e.g. direct immobilization or embedded polymeric shell.