Innovating Works

GLUGLU

Financiado
Deciphering the internalization profiles of mGlu receptors
G protein-coupled receptor (GPCR) are membrane proteins targeted by around 30 % of all marketed drugs. GPCR internalization is a mechanism that tunes the cell surface versus intracellular receptor ratio, a parameter that plays an... G protein-coupled receptor (GPCR) are membrane proteins targeted by around 30 % of all marketed drugs. GPCR internalization is a mechanism that tunes the cell surface versus intracellular receptor ratio, a parameter that plays an essentiG protein-coupled receptor (GPCR) internalization is a mechanism involved in the tuning of cell surface versus intracellular receptor ratio, a parameter that plays an essential role in controlling receptor physiological responses. This process is very well described for many GPCRs but surprisingly not for metabotropic glutamate receptors, mandatory dimeric GPCRs that are ubiquitously expressed in the brain to regulate neuronal transmission. Deciphering which are the internalization pathways related to mGlus emerge as a strategy for the regulation of their response to not only drugs but also endogenous neurotransmitters. I propose to examine the internalization profiles of mGlus in brain cells and to unravel the structural and molecular mechanisms involved in the process. I will use a biophysical technique optimized by the host team that allows the real-time and quantitative measurement of receptor internalization in both recombinant cells and brain cells, in combination with a microscopy approach that will complement the analysis. On top of that, I will use nanomolar range affinity nanobodies, single domain antibodies that have already been developed by the host team as a tool for ultimately elucidate which are the internalization profile of mGlus in brain cells. As a consequence of this project, the internalization profile of mGlus in neurons and astrocytes, and the cell machinery responsible for this process will be clarified for the first time. This knowledge will allow the fine-tuning of mGlus localization in the cell, as well as the validation of the nanobodies as a tool for studying endogenous mGlus. These outcomes will upgrade the current resources that the scientific community has to modulate mGlu receptor-mediated responses. ver más
31/08/2025
CNRS
196K€
Perfil tecnológico estimado
Duración del proyecto: 35 meses Fecha Inicio: 2022-09-12
Fecha Fin: 2025-08-31

Línea de financiación: concedida

El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2022-09-12
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
HORIZON-MSCA-2021-PF-01-01: MSCA Postdoctoral Fellowships 2021
Cerrada hace 3 años
Presupuesto El presupuesto total del proyecto asciende a 196K€
Líder del proyecto
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE... No se ha especificado una descripción o un objeto social para esta compañía.
Perfil tecnológico TRL 4-5