Combined Selective Plane Illumination Microscopy and Optical Projection Tomograp...
Combined Selective Plane Illumination Microscopy and Optical Projection Tomography for in vivo quantitative imaging of the developing zebrafish vasculature
"Recently conceived three-dimensional microscopy techniques are revolutionizing developmental biology and embryology studies, being able to visualize entire organisms in-vivo. Despite increasingly proposed imaging solutions, metho...
ver más
¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video
Proyectos interesantes
SmartMic
Smart Multimodal Microscopy for High Throughput Developmenta...
2M€
Cerrado
SPIM MOUSE
Characterization of the cellular mechanics of lineage segreg...
162K€
Cerrado
MOIPB
Multi modal optical imaging platform for Biology
195K€
Cerrado
INTOTOMORPHOGENESIS
in toto imaging of embryonic morphogenesis collective cell...
100K€
Cerrado
FIS2016-77892-R
IMAGEN OPTICA 3D ULTRARRAPIDA CON INFORMACION DE FASE EN MIC...
151K€
Cerrado
DEEP3P
Three photon fluorescence imaging deep inside scattering med...
185K€
Cerrado
Fecha límite de participación
Sin fecha límite de participación.
Descripción del proyecto
"Recently conceived three-dimensional microscopy techniques are revolutionizing developmental biology and embryology studies, being able to visualize entire organisms in-vivo. Despite increasingly proposed imaging solutions, methods suitable to quantify dynamic and mechanical parameters over a large region of a biological specimen are still lacking. This proposal aims to construct an optical system and a computational framework able to quantitatively study the developing embryonic zebrafish vasculature, looking at the interaction between gene expression and hemodynamic parameters, at high spatial resolution, over a large volume of the organism. The instrument will exploit an advanced version of Optical Projection Tomography, in order to provide a detailed three-dimensional map of the zebrafish cardiovascular system annotated with blood flow and shear stress parameters. Co-registered Selective Plane Illumination Microscopy images will enable the users to observe the expression of fluorescent markers at sub-cellular resolution and to correlate it to the hemodynamic/mechanical parameters. Low photo-bleaching and high acquisition speeds make this multimodal technology ideally suited for extended time-lapse experiments in live embryos. The system will be employed to characterize the development of the embryonic cardiovascular system in normal and mutant zebrafish embryos.
The researcher, proponent of this project, will be integrated in a highly multidisciplinary team and will be trained on microscopy and biological aspects of the research, being able to acquire new skills relevant for the development of his optical tomography laboratory and his career."