Innovating Works

3DchromArchaea

Financiado
Chromatin 3D architecture in Archaea
This proposal brings together the field of chromatin evolution and state-of-the-art structural biology to advance our understanding of a fundamental question: origin of chromatin structural and regulatory complexity. Eukaryotes an... This proposal brings together the field of chromatin evolution and state-of-the-art structural biology to advance our understanding of a fundamental question: origin of chromatin structural and regulatory complexity. Eukaryotes and most groups of archaea organize their genomes in the form of histone-based chromatin. Conservation of histones across the tree of life goes beyond protein sequence and histone fold. Tertiary arrangement of histones and DNA geometry in archaeal nucleosomes resemble those in eukaryotes; however, archaea can form special hypernucleosomes and slinky-like arrangements. Similarly to eukaryotes, some archaea have multiple histone variants and extended histone tails, although it is unclear whether their structural and regulatory roles are conserved. Eukaryotes inherited histone-based chromatin from archaea, however, the origins of eukaryotic chromatin complexity are enigmatic. Therefore, this proposal will address the 3D organization of chromatin in archaea to advance the understanding of chromatin evolution. We will test the following hypotheses: archaeal chromatin along with hypernucleosomes contains multiple open structures to maintain DNA accessibility and allow polymerase passage; histone variant exchange and histone tails in archaea play an important role in chromatin compaction similarly to eukaryotes. To test our hypotheses, we will synergistically apply state-of-the-art cryo-electron microscopy (cryo-EM) in situ and in vitro to selected archaeal systems. In situ cryo-EM will provide structural information about chromatin in native context, while cryo-EM of in vitro reconstituted chromatin will provide high-resolution structural information. Structural analysis complemented with biochemical, biophysical characterization and nucleosome positioning data will provide insights into 3D chromatin architecture in archaea in the context of eukaryotic chromatin evolution. ver más
31/03/2028
EMBL
1M€
Perfil tecnológico estimado
Duración del proyecto: 61 meses Fecha Inicio: 2023-02-02
Fecha Fin: 2028-03-31

Línea de financiación: concedida

El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2023-02-02
Línea de financiación objetivo El proyecto se financió a través de la siguiente ayuda:
ERC-2022-STG: ERC STARTING GRANTS
Cerrada hace 2 años
Presupuesto El presupuesto total del proyecto asciende a 1M€
Líder del proyecto
EUROPEAN MOLECULAR BIOLOGY LABORATORY No se ha especificado una descripción o un objeto social para esta compañía.