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Breaking the Resolution Limit in Two-Photon Microscopy Using Negative Photochrom...

Breaking the Resolution Limit in Two-Photon Microscopy Using Negative Photochromism Multiphoton microscopy is a benchmark tool in biomedical research, used for the fluorescence imaging in cellular environments. This has important implications for disease diagnosis and the monitoring of therapy response. In conven... ver más

28/02/2026

CHALMERS TEKNISKA...

2M€

Presupuesto del proyecto: 2M€

Líder del proyecto

CHALMERS TEKNISKA HOGSKOLA AB No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

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Financiación concedida El organismo HORIZON EUROPE notifico la concesión del proyecto el día 2022-11-01

HORIZON-EIC-2022-PATHFINDEROPEN-01-01: EIC Pathfinder Open 2022 Scope:You should apply to this call if you are looking for support from EIC Pathfinder Open to reali...

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Cerrada hace 2 años

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No hay información privada compartida para este proyecto. Habla con el coordinador.

4 Participantes

CHALMERS TEKNISKA HOGSKOLA A...

526.50K€ | Lider

UHU

299.13K€ | Participante

KU Leuven

817.75K€ | Participante

UGOT - GOETEBORGS UNIVERSITE...

622.75K€ | Participante

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Duración del proyecto: 39 meses Fecha Inicio: 2022-11-01
Fecha Fin: 2026-02-28

Líder del proyecto

CHALMERS TEKNISKA HOGSKOLA AB No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 2M€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Multiphoton microscopy is a benchmark tool in biomedical research, used for the fluorescence imaging in cellular environments. This has important implications for disease diagnosis and the monitoring of therapy response. In conventional two-photon microscopy the fluorescence intensity of the employed molecular probe is proportional to the square of the excitation light intensity, implying that the fluorescence from the sample is confined around the focal point, yielding good spatial resolution. The spatial resolution can be dramatically improved by drawing on higher-order processes such as four-photon absorption. However, its practical implementation imposes major technical challenges, such as extreme laser intensities in the deep NIR. For this reason four-photon microscopy has so far attracted academic interest only. The present proposal addresses this issue and provides a multidisciplinary answer to the question: Can we develop a technique that offers spatial resolution of four-photon microscopy, but relies on two-photon absorption? This would combine the upsides of two-photon microscopy (low excitation energies provided by standard lasers at around 800 nm, high penetration depth in tissue) with the superior spatial resolution of four-photon microscopy. The result would be nothing less than a true paradigm shift in multiphoton microscopy. The key to tackle this highly ambitious task lies in the design of molecules that combine two mechanistically entangled two-photon processes (4for2) for the generation of a fluorescence output. This is possible by merging two-photon absorption, two-photon FRET-induced photoisomerization, and negative photochromism. Purposefully designed switchable fluorophores, that unify these photophysical assets, will be developed and their performance will be critically validated in a multi-angle spectroscopic work-flow, including the demonstration in application-relevant biological environments.

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