Hola,
¿eres nuevo aquí?

Regístrate gratis y conecta tu empresa con financiación pública, partners y proyectos.

Tengo cuenta

Regístrate

Ver video

SPLICEOSACT

Financiado

Cerrado

Biochemical and CryoEM studies of spliceosome activation.

Pre-mRNA splicing is a fundamental maturation step of eukaryotic mRNAs that consists of the removal of introns and the concomitant ligation of exons by two successive transesterification reactions. This complex biological process... ver más

28/02/2021

UKRI

195K€

Presupuesto del proyecto: 195K€

Líder del proyecto

UNITED KINGDOM RESEARCH AND INNOVATION No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Fecha límite participación Sin fecha límite de participación.

Financiación concedida El organismo H2020 notifico la concesión del proyecto el día 2021-02-28

MSCA-IF-2017: Individual Fellowships Objective:The goal of the Individual Fellowships is to enhance the creative and innovative potential...

I+D

Cerrada

0% 100%

Características del participante

Este proyecto no cuenta con búsquedas de partenariado abiertas en este momento.

Información adicional privada

No hay información privada compartida para este proyecto. Habla con el coordinador.

1 Participantes

UKRI

195.45K€ | Lider

Conecta tu I+D

¿Tienes un proyecto y buscas un partner? Gracias a nuestro motor inteligente podemos recomendarte los mejores socios y ponerte en contacto con ellos. Te lo explicamos en este video

Duración del proyecto: 34 meses Fecha Inicio: 2018-04-03
Fecha Fin: 2021-02-28

Líder del proyecto

UNITED KINGDOM RESEARCH AND INNOVATION No se ha especificado una descripción o un objeto social para esta compañía.

TRL 4-5

Presupuesto del proyecto 195K€

Fecha límite de participación Sin fecha límite de participación.

Descripción del proyecto Pre-mRNA splicing is a fundamental maturation step of eukaryotic mRNAs that consists of the removal of introns and the concomitant ligation of exons by two successive transesterification reactions. This complex biological process is catalyzed by the spliceosome, a gigantic ribonucleoprotein particle that assembles de novo on each intron and uses a single RNA-based active site to perform both reactions. The spliceosome is composed of five snRNPs (U1, U2, U4, U5, U6) that are recruited to pre-mRNAs in a stepwise manner. When a pre-catalytic spliceosome, consisting of all five snRNPs, is formed on a pre-mRNA it has no pre-existing active site and undergoes extensive compositional and conformational changes to become catalytically competent. During this transition, two snRNPs (U1 and U4) are lost and several new factors are recruited, ensuring the formation and stabilization of the active site as well as the correct positioning of the pre-mRNA’s reactive groups in the catalytic centre. Thanks to recent technological advances in Electron Microscopy (EM), a series of cryoEM structures of fully assembled active spliceosomes at atomic resolution have been solved in the host group and elsewhere, in the past two years. These structures, which have created much excitement in the RNA community, visualize the spliceosome during each step of the catalytic cycle and allow a mechanistic understanding of catalysis. However, due to the scarcity of high-resolution information on earlier complexes, many questions remain regarding spliceosome activation. My project aims at filling a gap in our understanding of pre-mRNA splicing by unraveling the molecular details of spliceosome activation. To that end, I will use a combination of biochemical characterization and cryoEM to study yeast spliceosome captured at discrete early stages of activation.

Conecta tu I+D

Entra hoy

¿Olvidé mi contraseña?

Financiación

Empresas

CTIs/Universidades

Proyectos

Investigadores