Adaptations of the ATP synthesis machinery in bacteria and archaea to conditions...
Adaptations of the ATP synthesis machinery in bacteria and archaea to conditions of extremeenergy limitation in the deep subsurface
Microbes in the deep sub-seafloor have recently been found to survive under unprecedented conditions of extreme energy limitation. I aim to identify microbes in these deep subsurface communities with central energy metabolism that...
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Información proyecto ATP_ADAPT_LOW_ENERGY
Líder del proyecto
AARHUS UNIVERSITET
No se ha especificado una descripción o un objeto social para esta compañía.
TRL
4-5
Presupuesto del proyecto
221K€
Fecha límite de participación
Sin fecha límite de participación.
Descripción del proyecto
Microbes in the deep sub-seafloor have recently been found to survive under unprecedented conditions of extreme energy limitation. I aim to identify microbes in these deep subsurface communities with central energy metabolism that is adapted to these conditions. A combination of single-cell genomics and metagenomics will be used to identify members of the microbial community adapted to low energy flux by use of Na+-based chemiosmotic ATP synthesis. Following sequencing, genes encoding c subunits of the ATP synthase from the deep subsurface will be synthesized and expressed in E. coli to determine ATP synthase c ring stoichiometry. This project represents a novel approach to microbial ecology and physiology in energy-limited environments with a framework that combines state-of-the-art biogeochemistry, molecular microbial ecology, and biochemistry.